Key Terms. Biology MCQs for Class 12 Chapter Wise with Answers PDF Download was Prepared Based on … The molecule is a double-stranded circle 4,361 base pairs in length. b)Baculovirus. Have a glance at the MCQ of Chapter 11 Biology Class 12 and cross-check your answers during preparation. (a) PBR327 (b)PBR322 (c) both a above (d) None (18) Ti Plasmid is useful in (a) bringing new genes into animal cells (b) bringing new genes into plant cells (c) to nearly any sites on a chromosome (d) bringing tumour cells into plant cells (19) Many copies of a DNA molecule in a test tube are procurred by ‘p’ stands for ‘plasmid’, ‘B’ stands for Bolivar and ‘R’ stands for Rodriquez. These MCQ Questions on Biotechnology: Principles and Processes Class 12 with answers pave for a quick revision of the Chapter thereby helping you to enhance subject knowledge. c) Rhizopus nigricans. MCQ Questions; CBSE Notes; Biology MCQs for Class 12 with Answers Chapter 11 Biotechnology: Principles and Processes. (A) Bam HI (B) Eco RI (C) pBR322 (D) Hind III Answer :- (C) 2. • Examples : pBR322 plasmid pUC18 plasmid 11. This will help you to learn about the most frequently asked objective type questions on agriculture especially compiled for ICAR (JRF/SRF), ARS, IARI Ph.D and other competitive exams. Fusogen of protoplast culture is … Ori and rop; An antibiotic resistant gene. Here, you will discover the NEET Biology MCQ Questions for all Concepts as per the latest syllabus. DNA isolated from human placenta was used to construct two genomic libraries. A. The plasmid pBR322 contains genes for ampicillin resistance (Ap r) and tetracycline resistance (Tc r). b)organ transplantation. Gene Cloning and Human Genome Project Multiple Choice Questions and Answers 1. Practice more on a regular basis with these NEET Biology objective questions on air pollution and improve your subject knowledge & problem-solving skills along with time management. Any two restriction sites. Question: MCQ 10 Ampicillin Tetracycline PBR322 (4363 Bp) In A Molecular Cloning Experiment Using The Cloning Vector PBR322 Above, A Foreign DNA Insert Is Cloned Into The BamHl Site Of The Plasmid PBR322. Free PDF Download of CBSE Biology Multiple Choice Questions for Class 12 with Answers Chapter 11 Biotechnology: Principles and Processes. Maximum number of existing transgenic animals is of . EduRev is like a wikipedia just for education and the Vectors pBR322- PowerPoint Presentation, Biotechnology, Engg , Sem … 3. July 17, 2020 by Veerendra. d)Salmonella typhimurium. When a foreign DNA is inserted into the ampicillin resistance gene of pBR322, the plasmid is no longer able to confer resistance to ampicillin. {submitted by … N exposed to antibiotics that destroy bacteria without the plasmid. Select the correct answer . A. ; transcription: The synthesis of RNA under the direction of DNA. NEET Biology is the scoring paper in the medical entrance examination. (b) the creation of cells capable of synthesizing economically important molecules. The vector was mixed with the DNA sample described above, ligated, and recombinant plasmids were propagated in E. coli cells. Biology MCQ. Academia.edu is a platform for academics to share research papers. Plasmids in genetic engineering • In genetic engineering, plasmids provides a versatile tool that are used to make copies of particular genes. Answer and Explanation: 64. (a) PBR322 (b) R Plasmid types (c) CaMV195 (d) both a and b. The vector was named after the researchers who constructed it for the first time. List of 220 + multiple choice questions and answers on Agriculture! Test your knowledge of plasmids by using this interactive quiz. GK Que ( General knowledge questions and answers ) General Awareness and Current Affairs 2016 with Daily Current Affairs Quiz, Daily GK Quiz, News and Analysis for interview of IBPS Bank PO, IBPS Clerk , Railways, NDA, UPSC Exams, IAS, PCS and all other competitive Examinations. 229 likes. Draw a schematic sketch of pBR322 plasmid and label the following in it. pBR322 is a plasmid vector of E.coli extensively used for cloning purpose. Insertional Inactivation in pBR322 Insertional inactivation is a technique used in recombinant DNA technology and involves inactivation of a gene by inserting a fragment of DNA into the middle of its coding sequence. MCQ on Biotechnology and Genetic Engineering 1. ; expression vector: An expression vector, otherwise known as an expression construct, is generally a plasmid that is used to introduce a specific gene into a target cell. 9. Study the experimental protocol and solve the following multiple-choice questions! (a) Selectable markers in the coming vectors pBR322 : i. Ampicillin resistance antibioticsii.Tetracycline(b) The selection of recombinants due to inactivation of antibiotics is a difficult process and requires simultaneous plating on two plates having different antibiotics. (From the point of view of the aim of this experiment, … Which one of the following is used as vector for cloning genes into higher organisms? The purpose of a MCS in a plasmid is to allow a piece of DNA to be inserted into that region. [Delhi 2012]. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid. Fusogen of protoplast culture is … FIVE-CHOICE … a) sex determination . It contains the origin of replication(Ori) of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number. We are here for providing you MCQ on biology topics. Class 12th Biology Most VVI MCQ Objective Chapter Wise Question in English on New Pattern 12. (d) both b and c. 40. Class 12th Biology Most VVI MCQ Objective Chapter Wise Question in English on New Pattern 12. Plasmids am widely used as carriers of cloned genes, as for example the E. coli plasmid pBR322. Ans. In one of them (library A), the DNA was digested with EcoRI restriction endonuclease and mixed and ligated with EcoRI‐digested pBR322 plasmid DNA under conditions that allowed the joining of the sticky ends of plasmid DNA with genomic DNA fragments only. E.coli cloning vector pBR322 showing restriction sites (Hind III, Eco Rl Bam HI, Sal I, Pvu II, Pst I, Cla I), ori and antibiotic resistance genes (amp R and tet R). 1) pRK 2013 : 2) pBR 322 based plasmid vector : 3) all of these : 4) pRN 3 : 5) NULL : Complaint Here As Incorrect Question / Ans d)forensic science. Here, you will discover the NEET Biology MCQ Questions for all Concepts as per the latest syllabus. 8. (c) the efficient reduction of useful proteins. Coconut milk is used in tissue culture in which is present (a) cytokinin (b) auxin (c) gibberellin (d) ethylene. c)genetic engineering. The presence of the plasmid pBR322 in cells can be detected by plating potential transformants on an agar medium that contains either (or both) of ampicillin and tetracycline antibiotics. pBR322 DNA is a commonly used plasmid cloning vector in E. coli. [pBR322] 7 people answered this MCQ question is the answer among pBR322 for the mcq Which of the following is used as a vector for introducing a DNA fragment into human lymphocytes? B. Escherichia coli cells containing plasmid pBR322 DNA were incubated with the antitumor antibiotics bleomycin A2 (BLM A2) or talisomycin A (TLM A). TRUE . a)Retrovirus. 39. It is the first constructed cloning vector, created in 1977 in the laboratory of Herbert Boyer. Question 1. Where To Download Protein Engineering Mcq Protein Engineering Mcq As recognized, adventure as capably as experience about lesson, amusement, as well as contract can be gotten by just checking out a book protein engineering mcq along with it is not directly done, you could consent even more almost this life, concerning the world. Ti-plasmid derived Plant vectors: Disarmed Ti- plasmid vectors Ti plasmid disarmed Ti- plant vector . The left and right border sequences as well as the nopaline synthase gene of the Ti plasmid are conserved. KBC 9 … Which of the following is a plasmid ? FALSE. Contents: MCQs on Agronomy MCQs on Horticulture MCQs on Genetics MCQs on Soil Science MCQs on […] The main use of recombinant DNA technology are____ (a) production of transgenic humans. TRUE . This is done by inserting the gene to be replicated into the plasmid, then inserting the plasmids into bacteria by a “T ”. MCQ GK; Exam Quiz; old Exams; जीवनी ; नौकरी; विशेष; search; , -Question Answer MCQ Exam ON : Gene Transfer In Plants . BIOTECHNOLOGY AND ITS APPLICATION 1. 2.Satellie DNA is useful tool in . One of the most widely used plasmids in gene cloning in bacteria is pBR322, which has both resistance genes for ampicillin and tetracycline and many restriction sites. plasmid: A circle of double-stranded DNA that is separate from the chromosomes, which is found in bacteria and protozoa. Complete Vectors pBR322- PowerPoint Presentation, Biotechnology, Engg , Sem Notes | EduRev chapter (including extra questions, long questions, short questions, mcq) can be found on EduRev, you can check out lecture & lessons summary in the same course for Syllabus. (A) Bam HI (B) Eco RI (C) pBR322 (D) Hind III Answer :- (C) 2. For the construction of the genomic library, pBR322 was digested with EcoRI and the cohesive ends were removed and replaced by oligo(dG) molecules. 7 people answered this MCQ question is the answer among for the mcq DNA sequence of pBR322, having; pBR322 vector sequence. FALSE. AIIMS NEET Zoology Biotechnology Principles and Processes MCQ questions & solutions with PDF and difficulty level When plasmids are used as cloning vectors and carry a novel DNA sequence they are referred to as chimaeric plasmids. Which of the following is a plasmid ? pBR322 contains the genes for resistance to ampicillin and tetracycline and can be amplified with chloramphenicol. A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids. Any future products from the inactivated gene will not work because of the extra codes added to it. B. 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